一抗测试与滴定——比色法免疫染色(游离组织切片)
本服务包括组织制备、组织切片、采用不同稀释浓度的一抗进行比色法免疫染色、组织切片贴附、封片及载玻片标识。服务完成后,您将获得最多 30 张已完成比色法免疫染色的组织切片,可直接用于显微镜观察。此外,我们还将提供抗体滴定实验所采用的实验操作流程。
操作流程
Following cryoprotection, tissue will be rapidly frozen in isopentane pre-cooled to -70°C. The frozen tissue will then be cut on a cryostat and collected in our unique section cryoprotection solution (cf. Products, Cat. #PC101). Subsequently, sections will be processed free-floating for immunostaining with the specific antibody at various dilutions according to the avidin-biotin-complex (ABC) method¹ (for photo samples, cf. SI101).
备注
- 订购前请先申请报价。
- 研究人员需提供组织样本及特异性抗体
参考文献
- Hsu S. M., Raine L. and Fanger H. (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem. 29, 577-580.
