Tissue preparation & Golgi-Cox Staining with FD Rapid GolgiStain™ Kit
Golgi-Cox impregnation¹, ² has been one of the most effective techniques for studying both the normal and abnormal morphology of neurons, as well as glia. Using Golgi technique, subtle morphological alterations in neuronal dendrites and dendritic spines have been discovered in the brains of animals treated with drugs as well as in the postmortem brains of patients with neurological diseases³,⁴. However, the complex and time-consuming process of Golgi staining has been a major obstacle to the widespread application of this technique.
FD Rapid GolgiStain™ Kit (Cat. #PK401), designed based on the principle of the methods described by Ramón-Moliner², Glaser and Van der Loos⁵, has overcome most problems associated with the Golgi-Cox technique. The FD Rapid GolgiStain™ Kit has been tested extensively in the brains from several species of animals, as well as in specimens of the postmortem human brain. This kit has not only significantly simplified and improved the Golgi-Cox technique but has also proven to be extremely sensitive and reliable for demonstrating morphological details of neurons and glia, especially dendritic spines (cf. photo samples).
This service includes tissue preparation, sectioning, staining, coverslipping and slide labeling. As a result, you will receive up to all sections from each brain or block which are Golgi-Cox-impregnated and ready for microscopic observations.
Procedure
For details, see the User Manual for FD Rapid GolgiStain™ Kit (Cat. #PK401).
Remarks
- A quotation is required before placing an order.
- The investigator needs to provide tissue fixed with special fixative provided by FD NeuroTechnologies, Inc.
- Please contact us for more information.
References
- Corsi P. (1987) Camillo Golgi’s morphological approach to neuroanatomy. In Masland RL, Portera-Sanchez A and Toffano G (eds.), Neuroplasticity: a new therapeutic tool in the CNS pathology, pp 1-7. Berlin: Springer.
- Ramón-Moliner E. (1970) The Golgi-Cox technique. In Nauta WJH and Ebbesson SOE (eds.), Contemporary Methods in Neuroanatomy. pp 32-55, New York: Springer.
- Graveland GA, Williams RS, and DiFiglia M. (1985) Evidence for degenerative and regenerative changes in neostriatal spiny neurons in Huntington’s disease. Science. 227:770-3.
- Robinson TE, and Kolb B. (1997) Persistent structural modification in nucleus accumbens and prefrontal cortex neurons produced by previous experience with amphetamine. J. Neurosci. 17:8491-7.
- Glaser ME, and Van der Loos H. (1981) Analysis of thick brain sections by obverse-reverse computer microscopy: application of a new, high clarity Golgi-Nissl stain. J. Neurosci. Meth. 4:117-25.