Immunogold Labeling - 2 Primary Antibodies on Free-floating Sections
SKU: SI303
This service includes tissue preparation, sectioning, immunolabeling, mounting, coverslipping and labeling the slides. As a result, you will receive up to 60 immunolabeled sections per brain or per tissue block ready for microscopic observations.
Procedure
Following cryoprotection, tissue will be rapidly frozen in isopentane pre-cooled to -70°C. The frozen tissue will then be cut on a cryostat and collected in our unique section cryoprotection solution (cf. Products, Cat. #PC101). Subsequently, sections cut from various levels (or the levels of your choice) will be processed free-floating for immunostaining with 2 specific antibodies according to immunogold labeling technique¹ (cf. photo samples).
Remarks
- A quotation is required before placing an order.
- The investigator needs to provide fixed tissue and the specific antibodies.
- Please contact us for more information.
References
- Humbel BM, Sibon OCM, Stierhof YD, and Schwarz H. (1995) Ultra-small gold particles and silver enhancement as a detection system in immunolabeling and In
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