Immunofluorescence Labeling - 1 Primary Antibody on Free-floating Sections
SKU: SI201
This service includes tissue preparation, sectioning, immunostaining, mounting, coverslipping and labeling the slides. As a result, you will receive up to 60 immunostained sections per brain or per tissue block ready for microscopic observations.
Procedure
Following cryoprotection, tissue will be rapidly frozen in isopentane pre-cooled to -70°C. The frozen tissue will then be cut on a cryostat and collected in our unique section cryoprotection solution (cf. Products, Cat. #PC101). Subsequently, sections cut from various levels (or the levels of your choice) will be processed free-floating for immunostaining with 1 specific antibody according to the indirect immunofluorescence method¹ (cf. photo samples below).
Remarks
- A quotation is required before placing an order.
- The investigator needs to provide fixed tissue and the specific antibody.
- Please contact us for more information.
References
- Coons, A.H. (1958) Fluorescent antibody methods. In J.F. Danielli (ed): General Cytochemical Methods. New York: Academic Press, pp. 399-422.
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