Colorimetric Immunostaining - 2 Primary Antibodies on Free-Floating Sections
SKU: SI103
This service includes tissue preparation, sectioning, immunostaining, mounting, coverslipping and labeling of slides. As a result, you will receive up to 60 immunostained sections per brain or per tissue block ready for microscopic observations.
Procedure
Following cryoprotection, tissue will be rapidly frozen in isopentane pre-cooled to -70°C. The frozen tissue will then be cut on a cryostat and collected in our unique section cryoprotection solution (cf. Products, Cat. #PC101). Subsequently, sections cut through various levels (or the levels of your choice) will be processed free-floating for immunostaining with 2 specific antibodies according to the avidin-biotin-complex (ABC) method¹ (cf. photo samples).
Remarks
- A quotation is required before placing an order.
- The investigator needs to provide fixed tissue and the specific antibody.
- Please contact us for more information.
References
- Hsu S. M., Raine L. and Fanger H. (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem. 29, 577-580.
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