Testing & titration of primary antibody for immunofluorescence labeling on free-floating sections 

Cat. # SI403

This service includes tissue preparation, sectioning, immunolabeling with various dilutions of the primary antibody, mounting, coverslipping and labeling the slides. As a result, you will receive up to 30 immunostained tissue sections ready for microscopic observations. In addition, the experimental procedure used for the antibody titration will be provided.  

Procedure:  Following cryoprotection, tissue will be rapidly frozen in isopentane pre-cooled to -70°C. The frozen tissue will then be cut on a cryostat and collected in our unique section cryoprotection solution (cf. Products, Cat. #PC101). Subsequently, sections will be processed free-floating for immunostaining with the specific antibody at various dilutions according to the indirect immunofluorescence method¹ (for photo samples, cf. SI201).

Remarks:  

• A quotation is required before placing an order.

• The investigator needs to provide both tissue and the specific antibody.

Reference:  

1. Coons, A.H. (1958) Fluorescent antibody methods. In J.F. Danielli (ed): General Cytochemical Methods. New York: Academic Press, pp. 399-422.