Testing & titration of primary antibody for colorimetric immunostaining on free-floating sections 

Cat. # SI401 

This service includes tissue preparation, sectioning, immunolabeling with various dilutions of the primary antibody, mounting, coverslipping and labeling the slides. As a result, you will receive up to 30 immunostained tissue sections ready for microscopic observations. In addition, the experimental procedure used for the antibody titration will be provided.  

Procedure:  Following cryoprotection, tissue will be rapidly frozen in isopentane pre-cooled to -70�C. The frozen tissue will then be cut on a cryostat and collected in our unique section cryoprotection solution (cf. Products, Cat. #PC101). Subsequently, sections will be processed free-floating for immunostaining with the specific antibody at various dilutions according to the avidin-biotin-complex (ABC) method� (for photo samples, cf. SI101).

Remarks: 

• A quotation is required before placing an order.

• The investigator needs to provide both tissue and the specific antibody.

Reference:  

1. Hsu S. M., Raine L. and Fanger H. (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem. 29, 577-580.