Tissue preparation & immunofluorescence labeling with 3 primary antibodies on free-floating sections 

Cat. # SI205 

This service includes tissue preparation, sectioning, immunostaining, mounting, coverslipping and labeling the slides. As a result, you will receive up to 60 immunostained sections per brain or per tissue block ready for microscopic observations.

Procedure:  Following cryoprotection, tissue will be rapidly frozen in isopentane pre-cooled to -70°C. The frozen tissue will then be cut on a cryostat and collected in our unique section cryoprotection solution (cf. Products, Cat. #PC101). Subsequently, sections cut from various levels (or the levels of your choice) will be processed free-floating for immunostaining with 3 specific antibodies according to the indirect immunofluorescence method¹.

Remarks:  

• A quotation is required before placing an order.

• The investigator needs to provide fixed tissue and the specific antibodies.

• Please contact us for more information.

Reference: 

1. Coons, A.H. (1958) Fluorescent antibody methods. In J.F. Danielli (ed): General Cytochemical Methods. New York: Academic Press, pp. 399-422.