Tissue preparation & immunofluorescence labeling with 1 primary antibody on sections mounted on slides 

Cat. # SI202  

This service includes tissue preparation, sectioning, immunostaining, coverslipping and labeling the slides. As a result, you will receive up to 60 immunostained sections per brain or per tissue block ready for microscopic observations.  

Procedure:  Following cryoprotection, tissue will be rapidly frozen in isopentane pre-cooled to -70�C. The frozen tissue will then be cut on a cryostat and mounted on gelatin-coated microscope slides (cf. Products, Cat. #PO101). Subsequently, sections cut from various levels (or the levels of your choice) will be processed on slides for immunostaining with one specific antibody according to the indirect immunofluorescence method� (cf. photo samples below).
           Substance P-immunofluorescence in the spinal cord.  10 �m cryostat section was cut transversely from the chicken spinal cord. This section was processed on slide according to the indirect fluorescence method (for details, cf. J. Comp. Neurol. 278:253-264, 1988). Note substance P containing fibers mainly in the dorsolateral funiculus, Lissauer�s tract and the dorsal horn.
Substance P-immunofluorescence in the spinal cord.  An 10 �m cryostat section of the chicken spinal cord was processed as described above. Note dense substance P immunoreactivity in the dorsolateral funiculus and the dorsal horn.
Vasoactive intestinal polypeptide-immunofluo-rescence in the spinal cord.  An 10 �m cryostat section of the chicken spinal cord was processed on slide according to the indirect fluorescence method. Note 2 large neurons containing vasoactive intestinal polypeptide in the nucleus of the dorsolateral funiculus (for details, cf. J. Comp. Neurol. 278:253-264, 1988).


Remarks: 

  • A quotation is required before placing an order. 

  • The investigator needs to provide fixed tissue and the specific antibody.

  • Please contact us for more information.

Reference: 

  1. Coons, A.H. (1958) Fluorescent antibody methods. In J.F. Danielli (ed): General Cytochemical Methods. New York: Academic Press, pp. 399-422.