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Tissue
preparation & colorimetric immunostaining with 1 primary antibody on
sections mounted on slides Cat. # SI102
This
service includes tissue preparation, sectioning, immunostaining, mounting,
coverslipping and labeling the slides. As a result, you will receive up to 60
immunostained sections per brain or per tissue block ready for microscopic
observations. Procedure:
Following
cryopretection, tissue will be rapidly frozen in isopentane pre-cooled to
-70°C. The frozen tissue will then be cut on a cryostat and mounted on
gelatin-coated slides (cf. Products, Cat. #PO101). Sections cut from various levels (or the levels of your choice) will be processed on slides
for immunostaining with 1 specific antibody according to the
avidin-biotin-complex (ABC) methodą( cf. photo samples
below). |
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Cytokeratin 18-immunostained section counterstained with cresyl violet. This 12 µm frozen section of the rat prostate was processed for Cytokeratin 18-immunoreactivity (brown) and was then counterstained with FD cresyl violet solution (cf. Products, Cat. #PS102) (click to see enlarged photo). | ||
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CD31-immunostained section counterstained with hematoxylin. This 7 µm paraffin section of the mouse ear was processed for CD31-immunoreactivity (brown) and was then counterstained with FD hematoxylin solution (cf. Products, Cat. #PS104) (click to see enlarged photo). | ||
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Remarks:
Reference:
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