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Tissue
preparation & neurodegeneration detection with Gallyas silver staining
technique� in the brain Cat.
# SD102 This service includes tissue preparation, sectioning, silver-staining, mounting, coverslipping and labeling of slides. As a result, you will receive up to 40 silver-stained sections per brain or per tissue block ready for microscopic observations. Procedure:
Following
cryoprotection, tissue will be rapidly frozen in isopentane pre-cooled to
-70�C.
The frozen tissue will then be cut on a cryostat and collected in our unique
section cryoprotection
solution (cf. Products, Cat. #PC101).
Subsequently, sections cut through various levels (or the levels of your
choice) will be processed free-floating for the detection of neurons
undergoing degeneration with Gallyas silver staining technique�. This
technique was originally described
by Gallyas et al.�
and later modified�.
It is particularly useful for the detection of early neuronal injury in the central nervous system of experimental animals. Typically, both
the neuronal perikarya and processes are silver-stained. This technique
permits the morphological categorization of damaged neurons and the
detection of subtle changes in the morphology of cell bodies and processes
(cf. photo samples
below). |
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