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Tissue preparation & immunofluorescence labeling with 3 primary antibodies on sections mounted on slides

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Tissue preparation & immunofluorescence labeling with 3 primary antibodies on sections mounted on slides SI206Click Here to Request a Quote

This service includes tissue preparation, sectioning, immunostaining, coverslipping and labeling the slides. As a result, you will receive up to 60 immunostained sections per brain or per tissue block ready for microscopic observations.

Procedure:  Following cryoprotection, tissue will be rapidly frozen in isopentane pre-cooled to -70°C. The frozen tissue will then be cut on a cryostat and mounted on gelatin-coated microscope slides (cf. Products, Cat. #PO101). Subsequently, sections cut from various levels (or the levels of your choice) will be processed on slides for immunostaining with 3 specific antibodies according to the indirect immunofluorescence method¹.

Remarks: 

  • A quotation is required before placing an order.
  • The investigator needs to provide fixed tissue and the specific antibodies.
  • Please contact us for more information.

Reference:

  1. Coons, A.H. (1958) Fluorescent antibody methods. In J.F. Danielli (ed): General Cytochemical Methods. New York: Academic Press, pp. 399-422.