Tissue preparation & immunofluorescence labeling with 3 primary antibodies on free-floating sections
This service includes tissue preparation, sectioning, immunostaining, mounting, coverslipping and labeling the slides. As a result, you will receive up to 60 immunostained sections per brain or per tissue block ready for microscopic observations.
Procedure: Following cryoprotection, tissue will be rapidly frozen in isopentane pre-cooled to -70°C. The frozen tissue will then be cut on a cryostat and collected in our unique section cryoprotection solution (cf. Products, Cat. #PC101). Subsequently, sections cut from various levels (or the levels of your choice) will be processed free-floating for immunostaining with 3 specific antibodies according to the indirect immunofluorescence method¹.