Tissue preparation & neuronal apoptosis detection with in situ DNA nick end-labeling technique (TUNEL) in the brain
This service includes sectioning, TUNEL labeling, coverslipping and labeling of slides. As a result, you will receive up to 40 TUNEL-labeled sections per brain or per tissue block ready for microscopic observations.
Procedure: Unfixed frozen tissue will be cut on a cryostat and mounted on proteinase-resistant microscope slides (cf. Products, Cat. #PO102). Sections cut through various levels (or the levels of your choice) will then be processed on slides for the detection of neurons undergoing apoptosis with in situDNA nick end-labeling¹.
This technique, originally described by Gavrieli et al. (1992)¹, labels the nuclei of neurons undergoing DNA fragmentation. This method is based on the ability of terminal deoxynucleotidyl transferase to catalyze incorporation of biotinylated deoxyuridines onto the free 3'-hydroxyl termini of DNA fragments, which are considered as one of the most characteristic features of apoptosis², ³. The integrated biotins are amplified and visualized by the avidin-biotin-complex (ABC) method4 (cf. photo samples).