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Testing & titration of primary antibody for immunogold labeling on free-floating sections

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Testing & titration of primary antibody for immunogold labeling on free-floating sections SI405Click Here to Request a Quote

This service includes tissue preparation, sectioning, immunolabeling with various dilutions of the primary antibody, mounting, coverslipping and labeling the slides. As a result, you will receive up to 30 immunostained tissue sections ready for microscopic observations. In addition, the experimental procedure used for the antibody titration will be provided.

Procedure:  Following cryoprotection, tissue will be rapidly frozen in isopentane pre-cooled to -70°C. The frozen tissue will then be cut on a cryostat and collected in our unique section cryoprotection solution (cf. Products, Cat. #PC101). Subsequently, sections will be processed free-floating for immunostaining with the specific antibody at various dilutions according to the immunogold labeling technique¹ (for photo samples, cf. SI301).

Remarks:

  • A quotation is required before placing an order.
  • The investigator needs to provide both tissue and the specific antibody.

Reference:

  1. Humbel B. M., Sibon O. C. M., Stierhof Y.-D. and Schwarz H. (1995) Ultra-small gold particles and silver enhancement as a detection system in immunolabeling and In Situ Hybridization experiments. J. Histochem. Cytochem. 43, 735-737.