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Tissue preparation & immunogold labeling with 2 primary antibodies on sections mounted on slides

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Tissue preparation & immunogold labeling with 2 primary antibodies on sections mounted on slides SI304Click Here to Request a Quote

This service includes tissue preparation, sectioning, immunolabeling, coverslipping and labeling the slides. As a result, you will receive up to 60 immunolabeled sections per brain or per tissue block ready for microscopic observations.

Procedure:  Following cryoprotection, tissue will be rapidly frozen in isopentane pre-cooled to -70°C. The frozen tissue will be cut on a cryostat and mounted on gelatin-coated microscope slides (cf. Products, Cat. #PO101). Subsequently, sections cut from various levels (or the levels of your choice) will be processed on slides for immunostaining with 2 specific antibodies according to immunogold labeling technique¹.

Remarks:  

  • A quotation is required before placing an order.
  • Investigator needs to provide fixed (or unfixed frozen) tissue and the specific antibodies.
  • Please contact us for more information.

Reference:

  1. Humbel B. M., Sibon O. C. M., Stierhof Y.-D. and Schwarz H. (1995) Ultra-small gold particles and silver enhancement as a detection system in immunolabeling and In Situ Hybridization experiments. J. Histochem. Cytochem. 43, 735-737.