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Tissue preparation & colorimetric immunostaining with 2 primary antibodies on sections mounted on slides

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Tissue preparation & colorimetric immunostaining with 2 primary antibodies on sections mounted on slides SI104Click Here to Request a Quote

This service includes tissue preparation, sectioning, immunostaining, mounting, coverslipping and labeling the slide. As a result, you will receive up to 60 immunostained sections per brain or per tissue block ready for microscopic observations.

Procedure:  Following cryopretection, tissue will be rapidly frozen in isopentane pre-cooled to -70°C. The frozen tissue will then be cut on a cryostat and mounted on gelatin-coated slides (cf. Products, Cat. #PO101). Subsequently, sections cut from various levels (or the levels of your choice) will be processed on slides for immunostaining with 2 specific antibodies according to the avidin-biotin-complex (ABC) method¹.

Remarks: 

  • A quotation is required before placing an order.
  • The investigator needs to provide fixed (or unfixed frozen) tissue and the specific antibodies.
  • Please contact us for more information.

Reference:

  1. Hsu S. M., Raine L. and Fanger H. (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem. 29, 577-580.