|
FD
NeuroSilver™ Kit I
Cat.
# PK301
(for
300 sections)
Price: $495.60
Cat. # PK301A
(for
150 sections)
Price: $357.50
FD
NeuroSilver™ Kit I is designed for the detection of degenerating neurons
in fixed tissue sections of the central nervous system from experimental
animals. The principle of this kit is based on the findings that some
components of the neurons undergoing degeneration, such as lysosomes, axons, and
terminals, become particularly argyrophilic. Under certain conditions, these
cellular elements bind to the silver ions with high affinity. Upon reduction, the
silver ions form metallic grains that are visible under a light or electron
microscope.
FD
NeuroSilver™ Kit I has been used widely in animal studies under various
experimental conditions (cf. below for references using
this kit). This kit has proven extremely specific
and sensitive for the detection of degenerating neuronal somata, axons,
and terminals in both the brain and the spinal cord (cf. photo
samples).
It is particularly useful for the detection of small numbers of
degenerating neurons that may not be demonstrable with routine
histopathological techniques.
FD
NeuroSilver™ Kit I has also been proven to be very sensitive and reliable for the detection
of amyloid plaques in the brain of transgenic mice (cf. photo samples
). In addition, this kit may be used for
demonstrating neurodegeneration and/or amyloid plaques in tissue sections that have
been processed for immunohistochemistry (cf. photo
samples & also
Nature Neuroscience 3: 1301-1306, 2000). The
procedure of FD NeuroSilver™ Kit I takes approximately 1 hour.
Kit
Contents:
PK301A PK301
Solution A
250 ml 500 ml
Solution B
250 ml
500 ml
Solution C
250 ml x 2
500 ml x 2
Solution D
250 ml
500 ml
Solution E
1 ml
2 ml
Solution F
2 ml
3 ml
Solution G (5X)
500 ml 500 ml
Glass Specimen Retriever
2
2
User Manual 1
1
Materials
Required, but Not Included:
-
Double distilled or Milli-Q
water
-
6-well tissue culture plates
-
(Optional) Corning®
Netwells®
inserts (Corning Cat. #3480) and carrier kit (Corning Cat. #3521)
-
Histological supplies and
equipment, including microscope slides and coverslips,
natural hair brush, staining jars, xylene or xylene substitutes,
resinous
mounting medium (e.g. Permount®
and a light microscope.
Corning®
& Netwells®
are registered trademarks of Corning Inc.
Permount®
is a registered trademark of Fisher
Scientific.
User Manual
(PK301, Version
2006-1) 
References
using FD NeuroSilver™
kit:
-
Bliss RM, Hoffman
T, Finckbone V, Zeitouni T, Mansouri B and Strahlendorf J. AMPA-induced
excitotoxicity stimulates calpain activity and is exacerbated by tumor
necrosis factor-α; (TNF-α) in the CA1 and CA3 regions of the hippocampus
in an acute slice model. The FASEB Journal 21:943.2, 2007.
-
Kaur D,
Rajagopalan S, Chinta S, Kumar J, Monte DD, Cherny RA and Andersen JK.
Chronic ferritin expression within murine dopaminergic midbrain neurons
results in a progressive age-related neurodegeneration. Brain
Research 1140:188-194, 2007.
-
Setsuie R, Wang YL,
Mochizuki H, Osaka H, Hayakawa H, Ichihara N, Li H, Furuta A, Sano Y,
SunYJ, Kwon J, Kabuta T, Yoshimi K, Aoki S, Mizuno Y, Noda M and Wada K.
Dopaminergic neuronal loss in transgenic mice expressing the Parkinson’s
disease-associated UCH-L1 193M mutant. Neurochemistry International
50:119-129, 2007.
-
Wang A, Liang X, McAllister
JP, Li J, Brabant K, Black C, Finlayson P, Cao T, Tang H, Salley SO,
Auner GW, Simon Ng K.Y. Stability of and inflammatory response to
silicon coated with a fluoroalkyl self-assembled monolayer in the
central nervous system.
Journal of Biomedical Materials Research
81A:363-372, 2006.
-
Xu W, Wong TP, Chery N, Gaertner T,
Wang YT and Baudry M. Calpain-mediated mGluR1α truncation: A key step in
excitotoxicity.
Neuron 53:399-412, 2007.
-
Adhami
F, Liao GH, Morozov YM, Schloemer A,Schmithorst VJ, Lorenz JN, Dunn RS,
Vorhees CV Wills-Karp M, Degen JL, Davis RJ, Mizushima N, Rakic P,
Dardzinski BJ, Holland SK, Sharp FR and Kuan CY. Cerebral
ischemia-hypoxia induces in travascular coagulation and autophagy.
American J.
Pathology 169:566-583, 2006.
-
Cui L, Jeong H, Borovecki F,
Parkhurst CN, Tanese N and Krainc D. Transcriptional repression of
PGC-1α by mutant huntingtin leads to mitochondrial dysfunction and
neurodegeneration.
Cell
127:59-69, 2006.
-
Dow G, Bauman R, Caridha D,
Cabezas M, Du F, Gomez-Lobo R, Park M, Smith K and Cannard K. Mefloquine
induces dose-related neurological effects in a rat model.
Antimicrobial. Agents &
Chemotherapy 50:1045-1053, 2006.
-
Luo J,
Lin AH, Masliah E and Wyss-Coray T. Bioluminescence imaging of Smad
signaling in living mice shows correlation with excitotoxic neurode
generation. Proc.
Natl. Acad. Sci. USA 103(48):18326-31,
2006.
-
Martin LJ, Pan Y, Price AC,
Sterling W, Copeland NG,
Jenkins NA, Price DL and Lee MK.
Parkinson's disease α-synuclein transgenic mice develop neuronal mice
develop neuronal mitochondrial degeneration and cell death.
J. Neuroscience
26:41-50, 2006.
-
Rao MS,
Hattiangady B, Reddy DS and Shetty AK. Hippocampal neurodegeneration,
spontaneous seizures, and mossy fiber sprouting in the F344 rat model of
temporal lobe epilepsy.
J. Neurosci. Res. 83:1088-1105, 2006.
-
Saxon
DW, White G. Episodic vestibular disruption following ablation of the
inferior olive in rats: Behavioral correlates. Behavioural Brain
Research 175:128-138, 2006.
-
Saydoff
JA, Garcia RAG, Browne SE, Liu LS, Sheng J, Brenneman D, Hu Z, Cardin S,
Gonzalez A, von Borstel RW, Gregorio J, Burr H and Flint Beal M. Oral
uridine pro-drug PN401 is neuroprotective in the R6/2 and N171-82Q mouse
models of Huntington's disease.
Neurobiol. Dis.
24:455-465, 2006.
-
Theodore S, Stolberg S, Cass
WA and Maragos WF. Human immunodeficiency virus-1 protein Tat and
methamphetamine interactions.
Ann. N.Y.Acad. Sci. 1074:178-190, 2006.
-
Williams AJ, Hartings JA, Lu XCM, Rolli ML and Tortella FC. Penetrating
ballistic-like brain injury in the rat: differential time courses of
hemorrhage, cell death, in lammation, and remote degeneration.
J. Neurotrauma 23:1828-1846, 2006.
-
Yan X,
Benson JM, Gomez AP, Baden DG and Murray TF. Brevetoxin-induced neural
insult in the retrosplenial cortex of mouse brain.
Inhalation Toxicology
18:1109-1116, 2006.
-
Yazdani
U, German DC, Liang C-L, Manzino L, Sonsalla PK and Zeevalk GD. Rat
model of Parkinson's disease: chronic central delivery of
1-methyl-4-phenylpyridinium (MPP+).
Exp. Neurol.
200:172-183, 2006.
-
Zhang Y, Leavitt BR, van Raamsdonk
JM, Dragatsis I, Goldowitz D, MacDonald ME, Hayden MR and Friedlander
RM. Huntingtin inhibits caspase-3 activation.
EMBO Journal
25:5896-5906, 2006.
-
Liang ZQ, Wang XX, Wang Y,
Chuang DM, DiFiglia M, Chase TN and Qin ZH. Susceptibility of striatal
neurons to excitotoxic injury correlates with basal levels of Bcl-2 and
the induction of P53 and c-Myc immunoreactivity.
Neurobiol.
Dis. 20:562-573, 2005.
-
Williams AJ, Hartings JA, Lu
XCM, Rolli ML, Dave JR and Tortellla FC. Characterization of a new rat
model of penetrating ballistic brain injury.
J. Neurotrauma
22:313-331, 2005.
-
Zsombok A, Toth Z
and Gallyas F. Basophilia, acidophilia and argyrophilia of “dark”
(compacted) neurons during their formation, recovery or death in an
otherwise undamaged environment. Journal of Neuroscience Methods
142:145-152, 2005.
-
Abdel-Rahman A, Abou-Donia SM, El-Masry
EM, Shetty AK and Abou-Donia MB. Stress and combined exposure to low
doses of pyridostigmine bromide, deet, and permethrin produce
neurochemical and neuropathological alterations in cerebral cortex,
hippocampus, and cerebellum.
J. Toxicol. Environ. Health
Part A 67:163-192, 2004.
-
Lo Bianco C, Schneider BL, Bauer M, Sajadi A, Brice A,
Iwatsubo T and Aebischer P. Lentiviral vector delivery of parkin
prevents dopaminergic degeneration in an α–synuclein rat model of
Parkinson’s disease.
Proc. Natl. Acad. Sci. USA 101:17510-17515, 2004.
-
Paschen W, Yatsiv I, Shoham S and
Shoshami E. Brain trauma induces X-box protein 1 processing indicative
of activation of the endoplasmic reticulum unfolded protein response.
J.
Neurochem. 88:983-992, 2004.
-
Shoham S and Youdim MBH.
Nutritional iron deprivation attenuates ainate-induced neurotoxicity in
rats: implications for involvement of iron in neurodegerneration.
Ann. N.Y. Acad.
Sci. 1012:94-114, 2004.
-
Lauwers E, Debyser Z, Van Dorpe J,
De Strooper B, Nuttin B and Baekelandt V. Neuropathology and
neurodegeneration in rodent brain induced by lentivi ral vector-mediated
overespression of alpha-synclein.
Brain Pathol.
13:364-372, 2003.
-
Manning-Boğ AB, McCormack AL, Purisai MG, Bolin LM and Di
Monte A. α-synuclein overexpression protects against paraquat-induced
neurodegeneration. J.
Neuroscience 23: 3095-3099, 2003.
-
Shoham S, Bejar C, Kovalev E and Weinstock M. Intracerebroventricular injection of streptozotocin causes
neurotoxicity to myelin that contributes to spatial memory deficits in
rats. Exp. Neurol.
184: 1043-1052, 2003.
-
Sherer TB, Kim JH, Betarbet R and
Greenamyre JT. Subcutaneous rotenone exposure causes highly selective
dopaminergic degeneration and α-synuclein aggregation.
Exp. Neurol.
179:9-16, 2003.
-
Abdel-Rahman A, Shetty AK and
Abou-Donia MB. Disruption of the blood-brain barrier and neuronal cell
death in cingulate cortex, dentate gyrus, thalamus, and hypothalamus in
a rat model of gulf-war syndrome.
Neurobiol.
Dis. 10:306-326, 2002.
-
Bianco CL, Ridet JL, Schneider BL,
Déglon N and Aebischer P. α-synucleinopathy and selective dopaminergic
neuron loss in a rat lentiviral-based model of Parkinson’s disease.
Proc. Natl. Acad.
Sci. USA 99:10813-10818, 2002.
-
Ding Y, Yao B, Zhou Y, Park H,
McAllister JP and Diaz FG. Prereperfusion flushing of ischemic
territory: a therapeutic study in which histological and behavioral
assessments were used to measure ischemia-reperfusion injury in rats
with stroke.
J.
Neurosurg. 96:310-9, 2002.
-
Gordon MN, Holcomb LA, Jantzen PT,
DiCarlo G, Wilcock D, Boyett KW, Connor K, Melachrino J, O'Callaghan JP
and Morgan D. Time course of the development of Alzheimer-like pathology
in the doubly transgenic PS1+APP mouse.
Exp. Neurol.
173:183-195, 2002.
-
Guo-Ross SX, Clark S, Montoya DAC,
Jones KH, Obernier J, Shetty AK, White AM, Blusztajn JK, Wilson WA and
Swartzwelder HS. Prenatal choline supplementation protects against
postnatal neurotoxicity.
J. Neuroscience 22 RC195:1-6, 2002.
-
McCormack AL, Thiruchelvam M, Manning-Bog AB, Thiffault
C, Langston JW, Cory-Slechta DA and Di Monte DA. Environmental risk
factors and Parkinson’s disease: selective degeneration of nigral
dopaminergic neurons caused by the herbicide paraquat.
Neurobiol.
Dis. 10:119-127, 2002.
-
Montoliu C,
Humet M, Canales J-J, Burda J, Planells-Cases R, Sánchez-Baeza F,
Carbonell T, Pérez-Payá E, Messeguer A, Ferrer-Montiel A and Felipo V.
Prevention of in vivo excitotoxicity by a family of trialkylglycines, a
novel class of neuroprotectants.
J. Pharmacol. Exp. Ther.
301:29-36, 2002.
-
Tong W, Igarashi T, Ferriero DM
and Noble LJ. Traumatic brain injury in the immature mouse brain:
characterization of regional vulnerability.
Exp. Neurol.
176:105-116, 2002.
-
Ding Y, McAllister JP, Yao B, Yan
N and Canady AI. Neuron tolerance during hydrocephalus.
Neuroscience
106:659-667, 2001.
-
Ding Y, McAllister JP, Yao B, Yan
N and Canady AI. Axonal damage associated with enlargement of ventricles
during hydrocephalus: a silver impregnation study.
Neurol.
Res. 23:581-587, 2001.
-
Ding Y, Yao B, Lai Q and
McAllister JP. Impaired motor learning and diffuse axonal damage in
motor and visual systems of the rat following traumatic brain injury.
Neurol.
Res. 23:193-202, 2001.
-
Freeman A, Ciliax B, Bakay R,
Daley J, Miller RD, Keating G, Levey A and Rye D. Nigrostriatal
collaterals to thalamus degenerate in Parkinsonian animal models.
Ann Neurol.
50:321-329, 2001.
-
Liu ZP, Gastard M, Verina T, Bora
S, Mouton PR and Koliatsos VE. Estrogens modulate experimentally induced
apoptosis of granule cells in the adult hippocampus.
J. Comp. Neurol.
441:1-8, 2001.
-
Northington
FJ, Ferriero DM, Graham EM, Traystman RJ and Martin LJ. Early
neurodegeneration after hypoxia-ischemia in neonatal rat is necrosis
while delayed neuronal death is apoptosis.
Neurobiol.
Dis. 8:207-219, 2001.
-
Northington
FJ, Ferriero DM and Martin LJ. Neurodegeneration in the thalamus
following neonatal hypoxia-ischemia is programmed cell death.
Dev. Neurosci.
23:186-191, 2001.
-
Shoham S,
Javitt DC and Heresco-Levy U. Chronic high-dose glycine nutrition:
effects on rat brain cell morphology.
Biol. Psychiatry
49:876-885, 2001.
-
Zito MA,
Koennecke LA, McAuliffe MJ, McNally B, van Rooijen N and Heyes MP.
Depletion of systemic macrophages by liposome-encapsulated clodronate
attenuates striatal macrophage invasion and neurodegeneration following
local endotoxin infusion in gerbils.
Brain Res.
892:13-26, 2001.
-
Betarbet R,
Sherer TB, MacKenzie G, Garcia-Osuna M, Panov AV and Greenamyre JT.
Chronic systemic pesticide exposure reproduces features of Parkinson’s
disease. Nature
Neuroscience 3:1301-1306, 2000.
-
Kuhlmann AC
and Guilarte TR. Cellular and subcellular localization of peripheral
benzodiazepine receptors after trimethyltin neurotoxicity.
J. Neurochem.
74:1694-1704, 2000.
-
Sternfeld M,
Shoham S, Klein O, Flores-Flores C, Evron T, IdelsoGH, Kitsberg D,
Patrick JW and Soreq H. Excess “read-through” acetylcholinesterase
attenuates but the “synaptic” variant intensifies neurodeterioration
correlates. Proc.
Natl. Acad. Sci. USA 97:8647-8652, 2000.
-
Ahima RS,
Bjorbaek C, Osei S and Flier JS. Regulation of neuronal and glial
proteins by leptin: implications for brain development.
Endocrinology
140:2755-2762, 1999.
-
Burns LH, Jin Z and Bowersox SS.
The neuroprotective effects of intrathecal administration of the
selective N-type calcium channel blocker ziconotide in a rat model of
spinal ischemia.
J. Vasc. Surg. 30:334-343, 1999.
(Updated 7/13/2007)
|
|